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KMID : 0356920100580020153
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Korean Journal of Anesthesiology
2010 Volume.58 No. 2 p.153 ~ p.161
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Effect of propofol on cardiac function and gene expression after ischemic-reperfusion in isolated rat heart
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Kim Youn-Jin
Lim Hae-Ja
Choi Sung-Uk
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Abstract
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Background: The aim of this study was to examine the cardiac function and transcriptional response of the heart to propofol after ischemia-reperfusion.
Methods: Rat hearts were Langendorff-perfused using the modified Krebs-Henseleit buffer, and took 20 min stabilizing periods, 40 min ischemia periods, and then 120 min reperfusion period. The hearts were divided into 5 groups; Control: 180 min perfusion after stabilization, Ischemic: 40 min global ischemia after stabilization, followed by 120 min reperfusion, Pre: 2 μM propofol treatment was preformed only before ischemia, Post: 2 μM propofol treatment was performed only during reperfusion after ischemia, Pre/Post: 2 μM propofol treatment was performed both before and after ischemia. The measurement for cardiac performances, such as left ventricular developed pressure (LVDP), rate of left ventricular pressure generation (dP/dt), heart rate, and coronary flow were obtained. The expression profiles of isolated mRNA were determined by using Agilent microarray and real time-polymerase chain reaction (RT-PCR) was used to confirm the microarray results for a subset of genes.
Results: The Post group showed better LVDP and dP/dt than the Ischemic group. But there were no significant differences in heart rate and coronary flow among the groups. On the results of RT-PCR, the expressions of Abcc9, Bard1, and Casp4 were increased, but the expressions of Lyz, Casp8, and Timp1 were decreased in the Post group compared with the Ischemic group.
Conclusions: This study suggests that 2 μM propofol may provide cardioprotective effect, and modulate gene expression such as apoptosis, and KATP ion channel related-genes during reperfusion in the isolated rat hearts.
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KEYWORD
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Ischemia, Microarray, Propofol, Rat heart, Real time-polymerase chain reaction, Reperfusion
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